Molecular Geomicrobiology Lab
Most microorganisms in the environment are resistant to cultivation in the laboratory. The Molecular Geomicrobiology lab is equipped for the study of these microorganisms at the DNA, RNA and protein level. It includes:
- a FastPrep-24 high-speed benchtop homogenizer for extraction of nucleic acids and proteins from difficult environmental and mineral samples
- three AirClean 600 HEPA-filtered cabinets for sensitive DNA and RNA work
- two hybridization ovens for fluorescence in situ hybridization experiments
- a Beckman Coulter refrigerated centrifuge
- a Mini-PROTEAN system for running protein gels
- a Varian Cary 50 UV-Vis spectrophotometer with a microcell for quantifying DNA, RNA and microbial biomass and making measurements of colorimetric liquid assays
Other equipment for geomicrobiology includes ultra-cold (-80°C) and -20°C freezers, refrigerators, vortexers and a microcentrifuge.
Geochemistry and Microbiology Lab
Isolating and growing microorganisms in the laboratory is key to understanding how they interact with and transform minerals and their chemical environment. The Geochemistry and Microbiology lab is equipped for the cultivation of microorganisms (fungi, bacteria, etc.) and the preparation of media within a sterile environment. It includes:
- a Labconco Purifier Logic Class II Type A2 biosafety cabinet
- a Yamato SM-300 autoclave
- a Thermo-Barnstead Nanopure ultra-filtration water system
- a high-capacity shaking culture incubator.
The lab also contains equipment to digest rock and mineral samples in preparation for chemical analyses, including a chemical fumehood dedicated to hydrofluoric acid use, numerous hot plates, titration equipment and assorted Teflon and glassware.
General equipment for chemistry and geomicrobiology includes several chemical safety hoods, pH and conductivity meters, water baths, high-and low-temperature ovens, sonicator and high-precision balances.